| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 221 | Clostridium butyricum | TCTCCAGGAGTAAAGCTTGGA | TACCGCACCAGCTCCAATT | 58.10 | 59.32 | 258 | 102 |
100.00%
|
50.00%
|
| Primer 222 | Clostridium butyricum | GTGGAGCCATCATTGCTTTGA | ACAAACATTGCTGGGCTTGT | 59.18 | 58.88 | 193 | 102 |
100.00%
|
50.00%
|
| Primer 223 | Clostridium butyricum | TTCGCTGCGCTTATGGTCA | CCTCCACACCATCATAGCTGG | 60.08 | 60.20 | 269 | 102 |
100.00%
|
50.00%
|
| Primer 224 | Clostridium butyricum | AGGAAGTGGTTTGGGGAGT | GCCCTCCAACTCTGTCACTA | 58.04 | 58.73 | 236 | 102 |
100.00%
|
50.00%
|
| Primer 225 | Clostridium butyricum | ACTGCAGCTACAGGCCTTG | AGAGCCTCCTCTACAGCATCA | 60.00 | 60.06 | 236 | 102 |
100.00%
|
50.00%
|
| Primer 226 | Clostridium butyricum | TGGTGGTTCAAGTGTTGCAAC | ACAACTTCTTCGGCTCCACC | 59.80 | 60.25 | 234 | 101 |
100.00%
|
50.00%
|
| Primer 227 | Clostridium butyricum | TGGTGGTTCAAGTGTTGCAAC | ACCACAACTTCTTCGGCTCC | 59.80 | 60.25 | 237 | 101 |
100.00%
|
50.00%
|
| Primer 228 | Clostridium butyricum | AGGGAAAACCAGAACCGGAC | GGCTGAACAATATCTGGCACC | 59.89 | 59.32 | 154 | 101 |
100.00%
|
50.00%
|
| Primer 229 | Clostridium butyricum | TGGGCTTCCAAGGAACAAGG | AGAAGGAACCCCAGCGAAC | 60.18 | 59.62 | 207 | 101 |
100.00%
|
50.00%
|
| Primer 230 | Clostridium butyricum | CCATCTGGAAGCGGAAAGAC | TTCCAAGGCTTTCTGCTGC | 58.63 | 58.66 | 277 | 101 |
100.00%
|
50.00%
|